Difference between revisions of "Part:BBa K1033121"
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| − | <partinfo> | + | <partinfo>BBa_K1033121 short</partinfo> |
| − | Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". | + | Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes. |
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| − | This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes. | + | |
'''Applications''' | '''Applications''' | ||
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| − | + | '''Part improvement: team Tel-Hai 2017''' | |
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| + | Group: Tel-Hai 2017 | ||
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| + | Author: Almog Angel | ||
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| + | [https://parts.igem.org/Part:BBa_K2408023 BBa_K2408023] | ||
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| + | Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better | ||
| + | expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: <br>1. ADH1 - strong constitutive promoter for <I>S. cerevisiae</I>.<br> 2. His tag for purification. <br>3. ADH1 terminator for more effective expression. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K1033121 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K1033121 parameters</partinfo> |
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Latest revision as of 09:51, 29 October 2017
Miraculin
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Part improvement: team Tel-Hai 2017
Group: Tel-Hai 2017
Author: Almog Angel
Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better
expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features:
1. ADH1 - strong constitutive promoter for S. cerevisiae.
2. His tag for purification.
3. ADH1 terminator for more effective expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]