Difference between revisions of "Part:BBa K1033121"

Latest revision as of 09:51, 29 October 2017

Miraculin

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.

Part improvement: team Tel-Hai 2017

Group: Tel-Hai 2017

Author: Almog Angel

BBa_K2408023

Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features:
1. ADH1 - strong constitutive promoter for S. cerevisiae.
2. His tag for purification.
3. ADH1 terminator for more effective expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K1033120 short</partinfo>
+<partinfo>BBa_K1033121 short</partinfo>
-Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum", this part is a synthetic construct.
+Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
 '''Applications'''
@@ Line 9: / Line 9: @@
-==Characterization data from Uppsala iGEM 2013==
+'''Part improvement: team Tel-Hai 2017'''
-We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
+Group: Tel-Hai 2017
+Author: Almog Angel
+[https://parts.igem.org/Part:BBa_K2408023 BBa_K2408023]
+Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better
+expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: <br>1. ADH1 - strong constitutive promoter for  <I>S. cerevisiae</I>.<br> 2. His tag for purification. <br>3. ADH1 terminator for more effective expression.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K1033120 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K1033121 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1033120 parameters</partinfo>
+<partinfo>BBa_K1033121 parameters</partinfo>
 <!-- -->