Difference between revisions of "Part:BBa K1033121"
 
(15 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1033121 short</partinfo>
 
<partinfo>BBa_K1033121 short</partinfo>
  
−
None, this construct contains no RBS or promoter
+
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
  
−
== Results ==
+
'''Applications'''
−
We were successful in both subcloning the synthetic construct to a desired plasmid and isolating the Maraculin gene by itself. The sequencencing was done at GATC biotch and Uppsala Genome center. We were not successful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.  
+
 
 +
Miraculin can be used as a sweetener.
 +
 
 +
 
 +
'''Part improvement: team Tel-Hai 2017'''
 +
 
 +
Group: Tel-Hai 2017
 +
 
 +
Author: Almog Angel
 +
 
 +
 
 +
[https://parts.igem.org/Part:BBa_K2408023 BBa_K2408023]
 +
 
 +
 
 +
Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better
 +
expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: <br>1. ADH1 - strong constitutive promoter for  <I>S. cerevisiae</I>.<br> 2. His tag for purification. <br>3. ADH1 terminator for more effective expression.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:51, 29 October 2017

Miraculin

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.


Part improvement: team Tel-Hai 2017

Group: Tel-Hai 2017

Author: Almog Angel


BBa_K2408023


Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features:
1. ADH1 - strong constitutive promoter for S. cerevisiae.
2. His tag for purification.
3. ADH1 terminator for more effective expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]