Difference between revisions of "Part:BBa K1033121"
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Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better | Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better | ||
| − | expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: 1. ADH1 - strong constitutive promoter for <I>S. cerevisiae</I>. 2. His tag for purification 3. ADH1 terminator for more effective expression. | + | expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: <br>1. ADH1 - strong constitutive promoter for <I>S. cerevisiae</I>.<br> 2. His tag for purification <br>3. ADH1 terminator for more effective expression. |
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Revision as of 09:51, 29 October 2017
Miraculin
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Part improvement: team Tel-Hai 2017
Group: Tel-Hai 2017
Author: Almog Angel
Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better
expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features:
1. ADH1 - strong constitutive promoter for S. cerevisiae.
2. His tag for purification
3. ADH1 terminator for more effective expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]