Difference between revisions of "Part:BBa K2486009"

 
 
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<partinfo>BBa_K2486009 short</partinfo>
 
<partinfo>BBa_K2486009 short</partinfo>
  
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A part that generates TetR wen not represed by DtxR protein
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This part inverts the signal produced by the interaction between DtxR protein and the cognate operator.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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Wen Fe<sup>2+</sup> is added to a system where the DtxR protein is present (which can be achieved by the use of our [https://parts.igem.org/Part:BBa_K2486004 dtxR generator]) the protein enters in it's active state and is able to repress gene expression through its cognate operator.
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In our inverter system we have a modified constitutive promoter (BBa_X) that produces TetR in the absence of active DtxR. The produced TetR in turn can repress a second promoter with the tetR operator. In the presence of Fe<sup>2+</sup> the tetR expression would cease and permit reporter production.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2486009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2486009 SequenceAndFeatures</partinfo>

Latest revision as of 05:44, 29 October 2017


DtxR inverter using TetR

This part inverts the signal produced by the interaction between DtxR protein and the cognate operator.

Usage and Biology

Wen Fe2+ is added to a system where the DtxR protein is present (which can be achieved by the use of our dtxR generator) the protein enters in it's active state and is able to repress gene expression through its cognate operator. In our inverter system we have a modified constitutive promoter (BBa_X) that produces TetR in the absence of active DtxR. The produced TetR in turn can repress a second promoter with the tetR operator. In the presence of Fe2+ the tetR expression would cease and permit reporter production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 695
    Illegal NheI site found at 718
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]