Difference between revisions of "Part:BBa K2333434"
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This is an inducible mf-Lon assembled via gene blocks WM17_G107 and WM17_G108. We have modified the mf-Lon gene via codon-optimization for iGEM use and added a double terminator. | This is an inducible mf-Lon assembled via gene blocks WM17_G107 and WM17_G108. We have modified the mf-Lon gene via codon-optimization for iGEM use and added a double terminator. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This composite part is a combination circuit with the LacI repressor under the constitutive promoter J23105 and mf-Lon under the control of the PLlac 0-1 promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This IPTG-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements. | This composite part is a combination circuit with the LacI repressor under the constitutive promoter J23105 and mf-Lon under the control of the PLlac 0-1 promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This IPTG-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements. |
Revision as of 03:47, 29 October 2017
pLac0-1 mf-Lon
This is an inducible mf-Lon assembled via gene blocks WM17_G107 and WM17_G108. We have modified the mf-Lon gene via codon-optimization for iGEM use and added a double terminator.
Usage and Biology
This composite part is a combination circuit with the LacI repressor under the constitutive promoter J23105 and mf-Lon under the control of the PLlac 0-1 promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This IPTG-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3242
Illegal AgeI site found at 3326
Illegal AgeI site found at 3532
Illegal AgeI site found at 3557 - 1000COMPATIBLE WITH RFC[1000]