Difference between revisions of "Part:BBa K2333434"

 
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<partinfo>BBa_K2333434 short</partinfo>
 
<partinfo>BBa_K2333434 short</partinfo>
  
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This is an inducible mf-Lon assembled via gene blocks WM17_G107 and WM17_G108. We have modified the mf-Lon gene via codon-optimization for iGEM use and added a double terminator.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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This composite part is a combination circuit with the LacI repressor under the constitutive promoter J23105 and mf-Lon under the control of the PLlac 0-1 promoter. The mf-Lon protease specifically targets different protein degradation tags with varying affinities corresponding to varying degradation rates. This IPTG-inducible mf-Lon construct can be used in tandem with aTc-inducible pdt reporter constructs to obtain gene expression speed measurements.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:46, 29 October 2017


pLac0-1 mf-Lon

This is an inducible mf-Lon assembled via gene blocks WM17_G107 and WM17_G108. We have modified the mf-Lon gene via codon-optimization for iGEM use and added a double terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3242
    Illegal AgeI site found at 3326
    Illegal AgeI site found at 3532
    Illegal AgeI site found at 3557
  • 1000
    COMPATIBLE WITH RFC[1000]