Difference between revisions of "Part:BBa K2448008"

Revision as of 22:02, 28 October 2017

PsiR from Sinorhizobium meliloti

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium meliloti (SMb20483).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.

Our team used this transcription factor together with the Sinorhizobium meliloti pPsiA or pPsiR promoters (BBa_K2448014 and BBa_K2448015) to build two Psicose biosensors (BBa_K2448030 and BBa_K2448031). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 691
Illegal AgeI site found at 1024
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
+PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
 Our team used this transcription factor together with the ''Sinorhizobium meliloti'' pPsiA or pPsiR promoters ([[Part:BBa_K2448014|BBa_K2448014]] and [[Part:BBa_K2448015|BBa_K2448015]]) to build two Psicose biosensors ([[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]). The results show that PsiR behaved as predicted under and without induction.