Difference between revisions of "Part:BBa K2448006"

Revision as of 22:02, 28 October 2017

PsiR from Agrobacterium tumefaciens

This part is an E. coli codon optimized version of the PsiR found in Agrobacterium tumefaciens str. C58 (Atu4743).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.

Our team used this transcription factor together with the Agrobacterium tumefaciens pPsiA or pPsiR promoters (BBa_K2448010, BBa_K2448011) as well as with the pPsiTac1 synthetic promoter (BBa_K2448016) to build three Psicose biosensors (BBa_K2448025, BBa_K2448026 and BBa_K2448027). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 4
Illegal AgeI site found at 691
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
+PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
 Our team used this transcription factor together with the ''Agrobacterium tumefaciens'' pPsiA or pPsiR promoters ([[Part:BBa_K2448010|BBa_K2448010]], [[Part:BBa_K2448011|BBa_K2448011]]) as well as with the pPsiTac1 synthetic promoter ([[Part:BBa_K2448016|BBa_K2448016]]) to build three Psicose biosensors ([[Part:BBa_K2448025|BBa_K2448025]], [[Part:BBa_K2448026|BBa_K2448026]] and [[Part:BBa_K2448027|BBa_K2448027]]). The results show that PsiR behaved as predicted under and without induction.