Difference between revisions of "Part:BBa K2448015"
 
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<partinfo>BBa_K2448015 short</partinfo>
 
<partinfo>BBa_K2448015 short</partinfo>
  
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0.4 kb upstream of PsiR gene of Sinorhizobium meliloti (SMb20483)
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This part is the promoter region (0.4 kb upstream) of the PsiR gene of ''Sinorhizobium meliloti'' (SMb20483)
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pPsiR is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) which is inhibited in the presence of D-Psicose [1].
  
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===Usage and Biology===
 
===Usage and Biology===
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Our team used this pPsiR promoter together with the ''Sinorhizobium meliloti'' PsiR transcription factor ([[Part:BBa_K2448008|BBa_K2448008]]) to build a Psicose biosensor ([[Part:BBa_K2448031|BBa_K2448031]]). The results show that pPsiR is functional in ''E. coli'' and that it behaved as predicted under and without induction.
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Using the BPROM webserver [2], the predicted features of this promoter are:
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Threshold for promoters -  0.20
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Number of predicted promoters -      1
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Promoter Pos:    317 LDF-  3.58
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-10 box at pos.    302 gactatcat Score    53
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-35 box at pos.    281 ttgcac    Score    33
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Oligonucleotides from known TF binding sites:
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        lrp:  TTTCTTTT at position    273 Score -  9
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      ompR:  TTCTTTTT at position    274 Score -  8
  
 
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Latest revision as of 21:42, 28 October 2017


pPsiR from Sinorhizobium meliloti

This part is the promoter region (0.4 kb upstream) of the PsiR gene of Sinorhizobium meliloti (SMb20483)

pPsiR is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiR promoter together with the Sinorhizobium meliloti PsiR transcription factor (BBa_K2448008) to build a Psicose biosensor (BBa_K2448031). The results show that pPsiR is functional in E. coli and that it behaved as predicted under and without induction. 

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    317 LDF-  3.58
-10 box at pos.    302 gactatcat Score    53
-35 box at pos.    281 ttgcac    Score    33
Oligonucleotides from known TF binding sites:
       lrp:  TTTCTTTT at position     273 Score -   9
      ompR:  TTCTTTTT at position     274 Score -   8

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 179
  • 1000
    COMPATIBLE WITH RFC[1000]