Difference between revisions of "Part:BBa K2271105"
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<partinfo>BBa_K2271105 short</partinfo> | <partinfo>BBa_K2271105 short</partinfo> | ||
− | - | + | <h1>PEX5</h1> |
+ | <h2>Brief introduction</h2> | ||
+ | <p> | ||
+ | PEX5 is one of two proteins that mediate most of the protein import into the peroxisomal matrix. It recognizes the very c-terminal peroxisomal targeting signal 1 (<b>PTS1</b>), then folds itself to a more compact version and interacts with a variety of enzymes. | ||
+ | <br> | ||
+ | The whole process is divided into five steps: binding, transport, docking, translocation and receptor recycling. | ||
+ | </p> | ||
+ | |||
+ | <h2>Variant</h2> | ||
+ | <p> | ||
+ | We followed a targeted mutagenesis approach to achieve an orthogonal import mechanism − our PEX5 variant should recognize a non-native PTS1 variant which is not recognized with the wildtype PEX5. | ||
+ | </p> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 20:28, 28 October 2017
PEX5 variant R15
PEX5
Brief introduction
PEX5 is one of two proteins that mediate most of the protein import into the peroxisomal matrix. It recognizes the very c-terminal peroxisomal targeting signal 1 (PTS1), then folds itself to a more compact version and interacts with a variety of enzymes.
The whole process is divided into five steps: binding, transport, docking, translocation and receptor recycling.
Variant
We followed a targeted mutagenesis approach to achieve an orthogonal import mechanism − our PEX5 variant should recognize a non-native PTS1 variant which is not recognized with the wildtype PEX5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 409
- 1000COMPATIBLE WITH RFC[1000]