Difference between revisions of "Part:BBa K2448017"

 
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<partinfo>BBa_K2448017 short</partinfo>
 
<partinfo>BBa_K2448017 short</partinfo>
  
Promoter region (0.25 kb upstream) of FruB gene of Escherichia coli K-12 (ECK2162).
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This part is the promoter region (0.25 kb upstream) of the FruB gene of ''Escherichia coli'' K-12 (ECK2162).
  
 
pFruB is repressed by FruR transcription factor ([[Part:BBa_K2448009|BBa_K2448009]]) [1, 2].
 
pFruB is repressed by FruR transcription factor ([[Part:BBa_K2448009|BBa_K2448009]]) [1, 2].
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===Usage and Biology===
 
===Usage and Biology===
  
Our team used this pFruB promoter together with the E. coli FruR transcription factor ([[Part:BBa_K2448009|BBa_K2448009]]) to build a Fructose biosensor ([[Part:BBa_K2448032|BBa_K2448032]]). The results shows that pFruB behaved as predicted under and without induction.
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Our team used this pFruB promoter together with the ''E. coli'' FruR transcription factor ([[Part:BBa_K2448009|BBa_K2448009]]) to build a Fructose biosensor ([[Part:BBa_K2448032|BBa_K2448032]]). The results show that pFruB behaved as predicted under and without induction.
  
 
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Latest revision as of 20:01, 28 October 2017


pFruB from Escherichia coli

This part is the promoter region (0.25 kb upstream) of the FruB gene of Escherichia coli K-12 (ECK2162).

pFruB is repressed by FruR transcription factor (BBa_K2448009) [1, 2].

Usage and Biology

Our team used this pFruB promoter together with the E. coli FruR transcription factor (BBa_K2448009) to build a Fructose biosensor (BBa_K2448032). The results show that pFruB behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]