Difference between revisions of "Part:BBa K2448017"
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<partinfo>BBa_K2448017 short</partinfo> | <partinfo>BBa_K2448017 short</partinfo> | ||
− | pFruB | + | This part is the promoter region (0.25 kb upstream) of the FruB gene of ''Escherichia coli'' K-12 (ECK2162). |
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+ | pFruB is repressed by FruR transcription factor ([[Part:BBa_K2448009|BBa_K2448009]]) [1, 2]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | Our team used this pFruB promoter together with the ''E. coli'' FruR transcription factor ([[Part:BBa_K2448009|BBa_K2448009]]) to build a Fructose biosensor ([[Part:BBa_K2448032|BBa_K2448032]]). The results show that pFruB behaved as predicted under and without induction. | ||
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Latest revision as of 20:01, 28 October 2017
pFruB from Escherichia coli
This part is the promoter region (0.25 kb upstream) of the FruB gene of Escherichia coli K-12 (ECK2162).
pFruB is repressed by FruR transcription factor (BBa_K2448009) [1, 2].
Usage and Biology
Our team used this pFruB promoter together with the E. coli FruR transcription factor (BBa_K2448009) to build a Fructose biosensor (BBa_K2448032). The results show that pFruB behaved as predicted under and without induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]