Difference between revisions of "Part:BBa K2271105:Design"

 
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===Design Notes===
 
===Design Notes===
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This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it.
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We thereby provide a construct which allows full control over the peroxisomes protein contents.
  
  
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===Source===
 
===Source===
  
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Synthesized by IDT.
  
 
===References===
 
===References===

Revision as of 14:14, 28 October 2017


PEX5 variant R15


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is based on the yeasts PEX5 gene. In the course of a targeted mutagensis approach this PEX5 variant was designed to interact with a non-native PTS1 sequence. We verified that this variant does not detect the wildtype PTS1 but our designed PTS1* and leads to the import of the protein attached to it. We thereby provide a construct which allows full control over the peroxisomes protein contents.


Source

Synthesized by IDT.

References