Difference between revisions of "Part:BBa K2235005"
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Sialidase hydrolyses glycosidic linkages of terminal sialic acid residues in glycoproteins. In other words, it removes the sialic acids from the ends of the mucin constructs. This will help our bacteria to interact with the mucins for further degradation of the saccharides. Additionally, the removal of the sialic acids results in an exposure of other sugar groups on the mucins to other enzymes. Thus, the removal of sialic acids may result in even further mucus degradation by bacteria using different enzymes. | Sialidase hydrolyses glycosidic linkages of terminal sialic acid residues in glycoproteins. In other words, it removes the sialic acids from the ends of the mucin constructs. This will help our bacteria to interact with the mucins for further degradation of the saccharides. Additionally, the removal of the sialic acids results in an exposure of other sugar groups on the mucins to other enzymes. Thus, the removal of sialic acids may result in even further mucus degradation by bacteria using different enzymes. | ||
| − | + | [[File:SiaComp_Mechanism.png|400px|thumb|left|Figure 1: Schematic representation of Sialidase enzyme reaction mechanism.]] | |
| − | Figure | + | |
More details to the characterization of Sialidase can be found in the composite part BBa_K2235011. | More details to the characterization of Sialidase can be found in the composite part BBa_K2235011. | ||
Revision as of 10:25, 28 October 2017
Sialidase enzyme
Usage and Biology
Sialidase hydrolyses glycosidic linkages of terminal sialic acid residues in glycoproteins. In other words, it removes the sialic acids from the ends of the mucin constructs. This will help our bacteria to interact with the mucins for further degradation of the saccharides. Additionally, the removal of the sialic acids results in an exposure of other sugar groups on the mucins to other enzymes. Thus, the removal of sialic acids may result in even further mucus degradation by bacteria using different enzymes.
More details to the characterization of Sialidase can be found in the composite part BBa_K2235011.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 55
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 502
Illegal NgoMIV site found at 577
Illegal NgoMIV site found at 667 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1047