Difference between revisions of "Part:BBa K2446002:Design"

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===Design Notes===
 
===Design Notes===
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Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, whichis made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
+
Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012).

Latest revision as of 09:02, 28 October 2017


2_ZF54-8/43-8 binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.

Source

IDT (gBlocks)

References

Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012).