Difference between revisions of "Part:BBa K2235009:Design"

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===Design Notes===
 
===Design Notes===
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The following primers were used to amplify the gblock:
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Fwd: TATATGAATTCGCGGCCGCTTCTAGAGTAATACG
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Rev: ATATTCTGCAGCGGCCGCTACTAGTATTAGATGG
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His tag is N-terminally attached to the Sialidase enzyme coding sequence.
 
His tag is N-terminally attached to the Sialidase enzyme coding sequence.
  

Latest revision as of 08:29, 28 October 2017


Sialidase composite with T7 promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1119


Design Notes

The following primers were used to amplify the gblock:

Fwd: TATATGAATTCGCGGCCGCTTCTAGAGTAATACG

Rev: ATATTCTGCAGCGGCCGCTACTAGTATTAGATGG


His tag is N-terminally attached to the Sialidase enzyme coding sequence.

Source

References

Christensen, S. and Egebjerg, J. (2005), Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41: 225–231. doi:10.1042/BA20040144