Difference between revisions of "Part:BBa K2244006"
 
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ColE promoter contains a SOS operator sequence for the binding of LexA protein.
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ColE promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.
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===Usage and Biology===
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ColE promoter is 151bp in length. We used a light-repressed system containing a single light repressor LexA-VVD, and a ColE promoter that contains a SOS operator sequence for LexA binding to regulate the expression of target genes. The picture shows a colony PCR product of ColE promoter in pLEV1(408) vector. 
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<center><img src="https://static.igem.org/mediawiki/parts/6/68/Cole_jiaotu.png" style=" width:20%" /> </center>
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===Reference===
  
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Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35.
  
  

Latest revision as of 08:22, 28 October 2017

ColE promoter


ColE promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.


Usage and Biology

ColE promoter is 151bp in length. We used a light-repressed system containing a single light repressor LexA-VVD, and a ColE promoter that contains a SOS operator sequence for LexA binding to regulate the expression of target genes. The picture shows a colony PCR product of ColE promoter in pLEV1(408) vector.

Reference

Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]