Difference between revisions of "Part:BBa K2381012"
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<partinfo>BBa_K2381012 short</partinfo> | <partinfo>BBa_K2381012 short</partinfo> | ||
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It’s a gRNA plasmid which helps our CRISPR-System target to the MG1655 OriC sequence in order to achieve our expectation | It’s a gRNA plasmid which helps our CRISPR-System target to the MG1655 OriC sequence in order to achieve our expectation | ||
this year. Its backbone is taken from iGEM Kit Plate named BBa_K165005, but the BsaI restriction site which was in Amp. | this year. Its backbone is taken from iGEM Kit Plate named BBa_K165005, but the BsaI restriction site which was in Amp. | ||
Revision as of 06:28, 28 October 2017
3'-OriC gRNA
It’s a gRNA plasmid which helps our CRISPR-System target to the MG1655 OriC sequence in order to achieve our expectation
this year. Its backbone is taken from iGEM Kit Plate named BBa_K165005, but the BsaI restriction site which was in Amp. coding sequence has been deleted and a synonymous mutation has occurred by overlapping PCR. We used J23119 promoter, a high-intensity promoter, to obtain a higher expression of gRNA than dCas9. The 21bp-sequence comes after is a kind of sequence types taken from the article that is complementary to the Oric sequence and has a nice effect on our project. The full length gRNA scaffold is also designed after consulting literatures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]