Difference between revisions of "Part:BBa I746220:Experience"

Latest revision as of 21:55, 24 October 2007

It is not known whether this device works or not, since no reliable source of AIP was found.

The device was tested in various ways with the AIP generating device, I746210. Firstly, overnight cultures of recievers were diluted 1:100, grown for 2 hours and then induced with varying amounts of supernatant from overnight sender cultures. They were viewed after two hours on a fluorescence microscope. A variation on this was to induce and then use a plate reader to take readings. Finally, a lawn of recievers was prepared by diluting 100ul of culture into 3ml of molten top agar, and induction was by spotting supernatant or sender cell culture onto a filter paper disc in the centre of the lawn. None of these tests showed induction.

It was not determined which part of this system was faulty.

Applications of BBa_I746220

User Reviews

UNIQ9c4552af52157b99-partinfo-00000000-QINU UNIQ9c4552af52157b99-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
+It is not known whether this device works or not, since no reliable source of AIP was found.
+The device was tested in various ways with the AIP generating device, [https://parts.igem.org/wiki/index.php/Part:BBa_I746210 I746210]. Firstly, overnight cultures of recievers were diluted 1:100, grown for 2 hours and then induced with varying amounts of supernatant from overnight sender cultures. They were viewed after two hours on a fluorescence microscope. A variation on this was to induce and then use a plate reader to take readings. Finally, a lawn of recievers was prepared by diluting 100ul of culture into 3ml of molten top agar, and induction was by spotting supernatant or sender cell culture onto a filter paper disc in the centre of the lawn. None of these tests showed induction.
+It was not determined which part of this system was faulty.
 ===Applications of BBa_I746220===