Difference between revisions of "Part:BBa I739203"
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Plasmid pCK01BB1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.<br>
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[[Image:Pck01 Andy.jpg|350px]]
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In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], a MCS harbouring a SpeI site was introduced by PCR:<br>
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[[Image:PCK01-2.jpg]]<br>
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This MCS is the only difference between pCK01BB1 and pCK01. The term "BB1" stands for BioBrick version 1.
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===Purpose===
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<p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains... </p>
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=== References ===
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<p>
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[1]  Fernández S, de Lorenzo V, Pérez-Martín J <i>"Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains"</i>, Mol Microbiol. 16(2):205-13, 2000</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_I739202 parameters</partinfo>
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<partinfo>BBa_I739203 parameters</partinfo>
 
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Revision as of 21:39, 24 October 2007

pBR322BB2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
    Illegal NotI site found at 3612
    Illegal NotI site found at 3645
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3433
    Illegal SapI site found at 2350