Difference between revisions of "Part:BBa K2360007"
(→‎Lysis gene expression)
 
(8 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
 
It is special operon which can make a specific reponse to chromium ions.
 
It is special operon which can make a specific reponse to chromium ions.
−
 
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
−
===pChrB-ChrB===
 
−
A chromium sensing metal, E.coli-optimized
 
  
 
<!-- -->
 
<!-- -->
Line 14: Line 9:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
  
−
<partinfo>BBa_K2360003 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2360007 SequenceAndFeatures</partinfo>
  
  
  
−
<br>
+
 
−
[[File:SCUT_A gene.PNG]]<br>
+
 
 +
 
 +
<br>[[File:SCUT_A gene.PNG]]<br>
 
Figure 1. Bio-circuit of BBa_K2360007
 
Figure 1. Bio-circuit of BBa_K2360007
 
===Experiment===
 
===Experiment===
Line 25: Line 22:
 
We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions.
 
We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions.
 
Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.
 
Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.
−
[[File:SCUT_A PART Cr.PNG]]
+
<br>
 +
[[File:SCUT_A PART CR.PNG]]
 
<br>Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions.
 
<br>Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions.
 
<br/>
 
<br/>
 
we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr<sup>6+</sup>, which showed this devices did not work as we expected.  
 
we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr<sup>6+</sup>, which showed this devices did not work as we expected.  
−
<!-- -->
 
  
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K2360007 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 03:12, 28 October 2017


pChrB-ChrB

It is special operon which can make a specific reponse to chromium ions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 911
  • 1000
    COMPATIBLE WITH RFC[1000]





SCUT A gene.PNG
Figure 1. Bio-circuit of BBa_K2360007

Experiment

Lysis gene expression

We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions. Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.
SCUT A PART CR.PNG
Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions.
we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr6+, which showed this devices did not work as we expected.