Difference between revisions of "Part:BBa K2360007"

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	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>
−	<partinfo>BBa_K2360003 SequenceAndFeatures</partinfo>	+	<partinfo>BBa_K2360007 SequenceAndFeatures</partinfo>
−	<br>	+	<br>[[File:SCUT_A gene.PNG]]<br>
−	[[File:SCUT_A gene.PNG]]<br>	+
	Figure 1. Bio-circuit of BBa_K2360007		Figure 1. Bio-circuit of BBa_K2360007
	===Experiment===		===Experiment===

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Revision as of 02:49, 28 October 2017

pChrB-ChrB

It is special operon which can make a specific reponse to chromium ions.

Sequence and Features

Figure 1. Bio-circuit of BBa_K2360007

Experiment

Lysis gene expression

We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions. Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function. File:SCUT A PART Cr.PNG
Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions.
we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr⁶⁺, which showed this devices did not work as we expected.

Sequence and Features