Difference between revisions of "Part:BBa K2272019"

Revision as of 02:23, 28 October 2017

pVHb-HAO-Cyt

Construction intermediate/device with pVHb promter.

Usage and Biology

A improved version of https://parts.igem.org/Part:BBa_K1067002 and https://parts.igem.org/Part:BBa_K1067004 by combining the two coding sequences, which were suppose to work hand in hand, together. The VHb promoter was inserted to regulate the nitrification circuit expression under certain hypoxic conditions usually found in Sewage tanks. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 484
Illegal XhoI site found at 2048
Illegal XhoI site found at 4257
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 500
Illegal NgoMIV site found at 977
Illegal NgoMIV site found at 981
Illegal NgoMIV site found at 2749
Illegal NgoMIV site found at 3139
Illegal NgoMIV site found at 3406
Illegal NgoMIV site found at 3454
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 404
Illegal BsaI.rc site found at 452
Illegal BsaI.rc site found at 1019
Illegal BsaI.rc site found at 3179
Illegal BsaI.rc site found at 4856
Illegal BsaI.rc site found at 5012

@@ Line 5: / Line 5: @@
 Construction intermediate/device with pVHb promter.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+A improved version of https://parts.igem.org/Part:BBa_K1067002 and https://parts.igem.org/Part:BBa_K1067004 by combining the two coding sequences, which were suppose to work hand in hand, together. The VHb promoter was inserted to regulate the nitrification circuit expression under certain hypoxic conditions usually found in Sewage tanks.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2272019 SequenceAndFeatures</partinfo>