Difference between revisions of "Part:BBa K2305016"

Latest revision as of 02:16, 28 October 2017

GFP with cycle amplification system

Plux promoter stars the circuit, and Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux and form a cyclic amplifier to achieve a higher GFP expression.

Agarose gel electrophoresis

We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

Characterization map

Fig.The Growth curve of E.coil.

Fig.The GFP curve of E.coil.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1525
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2204

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2305016 short</partinfo>
-luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux
+Plux promoter stars the circuit, and Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux and form a cyclic amplifier to achieve a higher GFP expression.
+==Agarose gel electrophoresis==
+We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
+[[Image:BIT Figure DNA sequencing K2305016.png|200px|left|K230500]]<br clear="all">
+==Characterization map==
+[[Image:BIT Figure map K2305016.png|400px|left|K230500]]<br clear="all">
+Fig.The Growth curve of E.coil.
+[[Image:BIT Figure map K2305016a.png|400px|left|K230500]]<br clear="all">
+Fig.The GFP curve of E.coil.
 <!-- Add more about the biology of this part here