Difference between revisions of "Part:BBa K2360000"

 
 
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<partinfo>BBa_K2360000 short</partinfo>
 
<partinfo>BBa_K2360000 short</partinfo>
  
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The SRRz gene codes maybe three protein S,R,Rz.The product of S gene would cause lesions on the cytoplasmic membrane through which the product coded by the R gene escapes to the periplasm and causes murein-degrading, while the Rz gene’s product may be an endopeptidase that can cleave the oligopeptide crosslinks in the peptidoglycan and/or between peptidoglycan and the outer membrane.
  
 
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Figure 1. Bio-circuit of BBa_K2360000
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===Usage and Biology===
 
===Usage and Biology===
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We use this part to make a high-efficiency biosensor. It can combine with many detecting module to make a detecting device.
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We have developed the metal detecting module (BBa_K2360003) . Combining the lysis module and detecting module, we have constructed  devices which can respond to mercury ion.
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Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.
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[[File:SCUT_A PART HG.PNG]]
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<br>Figure 1. the growth curve of mercury detecting device in different concentration of mercury ions.
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As shown in the growth curves of E. coli with mercury detecting device (figure 2), the growth rate of E. coli BL21 is decline obviously after adding the concentration of Hg<sup>2+</sup> from 10<sup>-5</sup>M to 10<sup>-7</sup>M, indicating this part's  high-efficiency of lysis.
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<div class="img-center">
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[[File:SCUT_A gene.png]]
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</div>
  
 
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Latest revision as of 01:35, 28 October 2017


SRRz+rrnb terminater

The SRRz gene codes maybe three protein S,R,Rz.The product of S gene would cause lesions on the cytoplasmic membrane through which the product coded by the R gene escapes to the periplasm and causes murein-degrading, while the Rz gene’s product may be an endopeptidase that can cleave the oligopeptide crosslinks in the peptidoglycan and/or between peptidoglycan and the outer membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]