Difference between revisions of "Part:BBa K2447013"
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In this construct, the temperature sensitive TlpA36 protein will be produced with the addition of a upstream promoter (as specified by the user), and depending on the temperatures, it will either form Tlpa36 dimers that are capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form (above 36 degrees) which makes the protein unable to repress the promoter.
 
In this construct, the temperature sensitive TlpA36 protein will be produced with the addition of a upstream promoter (as specified by the user), and depending on the temperatures, it will either form Tlpa36 dimers that are capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form (above 36 degrees) which makes the protein unable to repress the promoter.
  
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As a proof of concept that the temperature system is fully functional, we had constructed and characterised an [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2447014 BBa_K2447014 ITPG-inducible temperature system] and coupled it to a downstream GFP reporter.
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As a proof of concept that the temperature system is fully functional, we had constructed and characterised an [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2447014 ITPG-inducible temperature system] and coupled it to a downstream GFP reporter (BBa_K2447014).
  
 
For more information on our experimental result and characterization protocol, do click [http://2017.igem.org/Team:NUS_Singapore/Experiments here].
 
For more information on our experimental result and characterization protocol, do click [http://2017.igem.org/Team:NUS_Singapore/Experiments here].

Revision as of 01:06, 28 October 2017


Temperature-sensitive repressible system

In this construct, the temperature sensitive TlpA36 protein will be produced with the addition of a upstream promoter (as specified by the user), and depending on the temperatures, it will either form Tlpa36 dimers that are capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form (above 36 degrees) which makes the protein unable to repress the promoter.

As a proof of concept that the temperature system is fully functional, we had constructed and characterised an ITPG-inducible temperature system and coupled it to a downstream GFP reporter (BBa_K2447014).

For more information on our experimental result and characterization protocol, do click [http://2017.igem.org/Team:NUS_Singapore/Experiments here].

For more information on how do we improve the overall characterization process by automation, do click [http://2017.igem.org/Team:NUS_Singapore/Measurement here]. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 811
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]