Difference between revisions of "Part:BBa P1002"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_P1002 short</partinfo>
 
<partinfo>BBa_P1002 short</partinfo>
  
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Ampicillin resistance cassette for modular BioBricks vector construction.
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Ampicillin resistance cassette including promoter and coding sequence. It lacks a terminator.
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===Usage and Biology===
 
===Usage and Biology===
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Designed by Tom Knight by designing primers to amplify the existing cassette from vector pSB1A3-P1010.
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*Used for [[Help:Plasmids/Construction|modular BioBrick vector construction]] using BioBrick base vector [[Part:BBa_I51020|BBa_I51020]].
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===References===
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'''Engineering BioBrick vectors from BioBrick parts'''<br>
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''Journal of Biological Engineering,'' 2008 Apr 14;2:5<br>
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Reshma Shetty, Drew Endy, Tom Knight<br>
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[http://www.jbioleng.org/content/2/1/5 URL]<br>
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656099 GenBank EU496092]
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_P1002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_P1002 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_P1002 parameters</partinfo>
 
<partinfo>BBa_P1002 parameters</partinfo>
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<!-- -->
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[http://2017.igem.org/Team:Heidelberg, Team Heidelberg 2017] improved this part by insertion of a mutation, yielding a higher enzyme activity (see: <partinfo>BBa_K2398550</partinfo>).  <br>
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(<small>--[[User:moritz_p95|moritz_p95]] 22:12, 27.10.2017 (UTC)</small>)

Latest revision as of 22:49, 27 October 2017

ampicillin resistance cassette

Ampicillin resistance cassette including promoter and coding sequence. It lacks a terminator.


Usage and Biology

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656099 GenBank EU496092]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[http://2017.igem.org/Team:Heidelberg, Team Heidelberg 2017] improved this part by insertion of a mutation, yielding a higher enzyme activity (see: BBa_K2398550).
(--moritz_p95 22:12, 27.10.2017 (UTC))