Difference between revisions of "Part:BBa I751011:Design"
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===Design Notes=== | ===Design Notes=== | ||
1. Move BBa_S03816 in the downstream of pBR Biobrick Tokyo Standard plasmid. | 1. Move BBa_S03816 in the downstream of pBR Biobrick Tokyo Standard plasmid. | ||
+ | |||
2. Introduce pcI promoter (bought from oligo house) in the upstream of Tokyo Standard site of pBR standard plasmid. | 2. Introduce pcI promoter (bought from oligo house) in the upstream of Tokyo Standard site of pBR standard plasmid. | ||
Latest revision as of 16:52, 24 October 2007
pcI-lux-lac-mCherry on pBR Standard
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5120 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2762
Illegal SpeI site found at 5121
Illegal PstI site found at 5135
Illegal NotI site found at 2768
Illegal NotI site found at 5128 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2762
Illegal BglII site found at 2890
Illegal BglII site found at 3572
Illegal BamHI site found at 2798 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5121 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2762
Illegal XbaI site found at 2777
Illegal SpeI site found at 5121
Illegal PstI site found at 5135 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2003
Illegal SapI site found at 920
Design Notes
1. Move BBa_S03816 in the downstream of pBR Biobrick Tokyo Standard plasmid.
2. Introduce pcI promoter (bought from oligo house) in the upstream of Tokyo Standard site of pBR standard plasmid.