Difference between revisions of "Part:BBa K2305008"
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[[Image:BIT Figure DNA sequencing K2305008a.png|200px|left|K230500]] <br clear="all"> | [[Image:BIT Figure DNA sequencing K2305008a.png|200px|left|K230500]] <br clear="all"> | ||
==Characterization map== | ==Characterization map== | ||
| − | [[ | + | [[Image:BIT Figure map K2305008.png|400px|left|K230500]]<br clear="all"> |
Fig.The Growth curve of E.coil. | Fig.The Growth curve of E.coil. | ||
Latest revision as of 22:20, 27 October 2017
pcat promoter and plux,luxr generator
pcat promtor controlled plux and LuxR
Agarose gel electrophoresis
We calculated the new part’s base number to be 928bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing
The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
Characterization map
Fig.The Growth curve of E.coil.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 64