Difference between revisions of "Part:BBa K2305008"

 
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pcat promtor controlled plux and LuxR
 
pcat promtor controlled plux and LuxR
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==Agarose gel electrophoresis==
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We calculated the new part’s base number to be 928bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
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[[Image:BIT Figure DNA sequencing K2305008.png|200px|left|K230500]]<br clear="all">
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==DNA sequencing==
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The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
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[[Image:BIT Figure DNA sequencing K2305008a.png|200px|left|K230500]] <br clear="all">
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==Characterization map==
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[[Image:BIT Figure map K2305008.png|400px|left|K230500]]<br clear="all">
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Fig.The Growth curve of E.coil.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:20, 27 October 2017


pcat promoter and plux,luxr generator

pcat promtor controlled plux and LuxR

Agarose gel electrophoresis

We calculated the new part’s base number to be 928bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing

The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500

Characterization map

K230500

Fig.The Growth curve of E.coil.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64