Difference between revisions of "Part:BBa K112805"
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Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. | Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. | ||
| + | |||
| + | Group: (Michigan 2017) | ||
| + | Author: (Aaron Renberg) | ||
| + | Summary: We improved this part by optimizing the codons for translation in E. coli using IDT’s codon optimization tool, and by eliminating the illegal XbaI site that Imperial College London’s 2011 team found, making it much easier for future iGEM teams to use. The changes we made were T358C, T556C, T563C, and T571C. | ||
| + | Link: https://parts.igem.org/Part:BBa_K2301000 | ||
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Revision as of 21:23, 27 October 2017
[T4 holin]
Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
Group: (Michigan 2017) Author: (Aaron Renberg) Summary: We improved this part by optimizing the codons for translation in E. coli using IDT’s codon optimization tool, and by eliminating the illegal XbaI site that Imperial College London’s 2011 team found, making it much easier for future iGEM teams to use. The changes we made were T358C, T556C, T563C, and T571C. Link: https://parts.igem.org/Part:BBa_K2301000
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]