Difference between revisions of "Part:BBa K2267030"

Revision as of 19:04, 27 October 2017

Plux3

Promoter activated by LuxR in concert with HSL

This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site. A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri. In vibrio fischeri, the lux box, which is a 20-base inverted repeat unit, is positioned 42.5 bases upstream of the transcriptional start of the lux operon and is required for transcriptional activation.

design

Quorum-sensing bacteria such as Vibrio fischeri, are able to detected their own population density and implement density-based decision-making，Using the luxI/luxR quorum-sensing system, synthetic biologists have designed a large number of devices in prokaryotic microorganisms.Inevitably, high performance is required from such devices, and this includes reliability, sensitivity, Hence, to improve the properties of population-density switches, we design of ultrasensitive responses use Point mutation NAME SITE HOW before after plux1 -8 "delete " C plux2 -7 " delete " G plux3 -10 substitute G A plux4 -10 substitute G T plux5 -11 substitute A T plux6 -12 substitute T C plux7 -12&-11 substitute TA CT plux8 -12&-10 substitute TAG CAA plux9 -11&-10 substitute AG TT plux10 -11&-10 substitute AG TA plux11 -12&-10 substitute TAG CAT plux12 -12&-11&-10 substitute TAG CTT plux13 -12&-11&-9 substitute TAG CTA

Sequence and Features