Difference between revisions of "Part:BBa K2382003"

Revision as of 18:03, 27 October 2017

T7 promoter & Lac operator and RBS from PET-29a

This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS. Sequence and Features

Assembly Compatibility:

This sequence has lac operator . When we want to use it to produce protein, it could be induced by lac operator.

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 <partinfo>BBa_K2382003 short</partinfo>
-This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.because the original part has the restriction site”Xba1”in the sequence,and the sequence of this restriction site is” TCTAGA. “ We do the single mutation to convert TC”T”AGA to TC”C”AGA . Thus, it would not effect role to make the composition of our part.
+This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===