Difference between revisions of "Part:BBa K2382003"

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<partinfo>BBa_K2382003 short</partinfo>
 
<partinfo>BBa_K2382003 short</partinfo>
  
This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.because the original part has the restriction site”Xba1”in the sequence,and the sequence of this restriction site is” TCTAGA. “ We do the single mutation to convert TC”T”AGA to TC”C”AGA . Thus, it would not effect role to make the composition of our part.
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This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS.
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:03, 27 October 2017


T7 promoter & Lac operator and RBS from PET-29a

This part originated from pET-29 a (+) Vectors and Part:BBa_K525998. It is composed of T7 promoter, Lac operator, and RBS. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has lac operator . When we want to use it to produce protein, it could be induced by lac operator.