Difference between revisions of "Part:BBa K2305001"
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Promoter plux star the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
 
Promoter plux star the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
 +
 +
==Agarose gel electrophoresis==
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We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 +
 +
[[Image:BIT Figure Electrophoretogram K2305001-a.png|200px|left|K230501a]] [[Image:BIT Figure Electrophoretogram K2305001-b.png|200px|left|K230501b]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
  
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==DNA sequencing==
 
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The figure is a comparison with the result of the sequencing. It verified the accuracy of the sequence further.
 
  
  

Revision as of 17:25, 27 October 2017


cycle amplification system and GFP/RFP dual fluorescent system

Promoter plux star the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.

Agarose gel electrophoresis

We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230501a
K230501b

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1525
    Illegal BglII site found at 2704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 4497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383