Difference between revisions of "Part:BBa K2275005"
 
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<partinfo>BBa_K2275005 short</partinfo>
 
<partinfo>BBa_K2275005 short</partinfo>
  
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We add an extra NsrR binding sequence before RBS which is expected to decrease the basal level of expression on the original part of K381001 in order to improve function of BBa_K381001.
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We add an extra NsrR binding sequence at the upstream of RBS on the original part of K381001 in order to lower the basal level of expression of GFP.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:05, 27 October 2017

PyeaR(BBa_K216005) with additional NsrR binding site in front of B0030 RBS

We add an extra NsrR binding sequence at the upstream of RBS on the original part of K381001 in order to lower the basal level of expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 107
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]