Difference between revisions of "Part:BBa K2382010"
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<span class='h3bb'>Sequence and Features</span>
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By ligating the constitutive promoter (BBa_J23101), strong ribosome
 
By ligating the constitutive promoter (BBa_J23101), strong ribosome
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RFP as indicator, and make their own test strip!
 
RFP as indicator, and make their own test strip!
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
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===Usage and Biology===
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<partinfo>BBa_K2382010 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 16:54, 27 October 2017

Anti-aflatoxin scFv fusion protein construction DNA sequence

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1342
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 403
    Illegal SapI.rc site found at 316


By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator, and make their own test strip!


Usage and Biology