Difference between revisions of "Part:BBa K2305000"
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The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further. | The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further. | ||
| − | [[Image:BIT Figure DNA sequencing K2305000 . | + | [[Image:BIT Figure DNA sequencing K2305000.PNG|200px|left|K230500]]<br clear="all"> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:29, 27 October 2017
strong promoter, cycle amplification system and GFP/RFP dual fluorescent system
Strong promoter pcat and promoter plux start the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
Agarose gel electrophoresis
We calculated the new part’s base number to be 4739bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing
The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1591
Illegal BglII site found at 2770 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4451
Illegal AgeI site found at 4563 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 64
Illegal BsaI.rc site found at 3449