Difference between revisions of "Part:BBa K2205004"

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===Usage and Biology===
 
  
<div class="result_img SOX"><img class="result_img" src="https://static.igem.org/mediawiki/2017/4/4b/T--Newcastle--SOX_testing.JPG" width="100%"/>
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<img class="result_img" src="https://static.igem.org/mediawiki/2017/4/4b/T--Newcastle--SOX_testing.JPG" width="100%"/>
 
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<p class="legend"><strong>Figure 3 Testing of Sarcosine Oxidase with Sarcosine:</strong> Lysed cell cultures tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.</p>
 
<p class="legend"><strong>Figure 3 Testing of Sarcosine Oxidase with Sarcosine:</strong> Lysed cell cultures tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.</p>
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Revision as of 16:29, 27 October 2017


T7-Sarcosine Oxidase

Sarcosine Oxidase under the control of an IPTG-inducible T7 promoter. Catalyses sarcosine to yield glycine, formaldehyde and hydrogen peroxide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]