Difference between revisions of "Part:BBa K2205002"

 
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<partinfo>BBa_K2205002 short</partinfo>
 
<partinfo>BBa_K2205002 short</partinfo>
  
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deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. This is an expression part where deGFP is cloned downstream of a strong Anderson promoter (J23100) and a strong RBS (B0034). deGFP is followed by a double terminator (B0015).
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deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.
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===CFPS Expression of deGFP===
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[[File:T--Newcastle--BB_CFPS_deGFP_sfGFP.png|500px|]]
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'''CFPS Expression of ''J23100-deGFP'' and ''J23100-sfGFP'' Constructs:''' Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
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===''In vivo'' Qualitative Comparison===
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[[File:T--Newcastle--BB_deGFP.png|400px|]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:29, 27 October 2017


J23100-deGFP

deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.


CFPS Expression of deGFP

T--Newcastle--BB CFPS deGFP sfGFP.png


CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs: Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.


In vivo Qualitative Comparison

T--Newcastle--BB deGFP.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]