Difference between revisions of "Part:BBa K2492000"

 
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<partinfo>BBa_K2492000 short</partinfo>
 
<partinfo>BBa_K2492000 short</partinfo>
  
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Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group we use it to locate the neuron AWB of C.elegans. If we get successfully microinjection C.elegans, GFP will specifically high expressed in AWB with the help of special promoter str1(no biobrick part).
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Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:21, 27 October 2017


GFP (optimised, for use in the C. elegans chassis)

Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797