Difference between revisions of "Part:BBa K2377005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | From Total silencing by intronspliced hairpin RNAs we learned that intron-hpRNA is more efficient than dsRNA. And we design this element to test the efficiency of different RNA structure. And the copy number of L4440 is below our expectation. Using hpRNA, we can improve our production rate. | |
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===Source=== | ===Source=== | ||
Latest revision as of 14:57, 27 October 2017
TrxzL, a developed element to generate trxz hpRNA with loop120
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 154
Illegal AgeI site found at 1159 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
From Total silencing by intronspliced hairpin RNAs we learned that intron-hpRNA is more efficient than dsRNA. And we design this element to test the efficiency of different RNA structure. And the copy number of L4440 is below our expectation. Using hpRNA, we can improve our production rate.
Source
arabidopsis thaliana