Difference between revisions of "Part:BBa K2302009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
===Results=== | ===Results=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 12:40, 27 October 2017
His＋Lpp-ompA＋L-FABP
this plasmid expresses a fatty acid binding protein that can be anchoredas a fused protein on the outer membrane. It consists of four basic parts: the T7 promoter, RBS, Lpp-OmpA, and L-FABP. Lpp-OmpA is a sequence encoding a functional peptide that allows surface anchoring of its fused FABP. FABP can bind long chain fatty acids. By transforming this plasmid, the host bacteria can catch long chain fatty acids in liquid and carry it on its outer membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 462
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 687