Difference between revisions of "Part:BBa K2226005"

 
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<partinfo>BBa_K2226005 short</partinfo>
 
<partinfo>BBa_K2226005 short</partinfo>
  
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c-Myc promoter, FRB rapamycin-binding domain (FRB) of human mTOR, fused to n-terminal of split luciferase (Nluc398), acts as one half of a promoter-reporter system, forms FRB-rapamycin-FKBP complex in presence of rapamycin.
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c-Myc promoter, FK-506-binding protein 12 fused to c-terminal of split luciferase (Cluc394), acts as one half of a promoter-reporter system, forms FRB-rapamycin-FKBP complex in presence of rapamycin.
  
 
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Revision as of 11:55, 27 October 2017


c-Myc promoter - FKBP - Cluc394

c-Myc promoter, FK-506-binding protein 12 fused to c-terminal of split luciferase (Cluc394), acts as one half of a promoter-reporter system, forms FRB-rapamycin-FKBP complex in presence of rapamycin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 45
    Illegal NheI site found at 68
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal AgeI site found at 827
  • 1000
    COMPATIBLE WITH RFC[1000]