Difference between revisions of "Part:BBa K2492002"
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[[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]] | [[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 11:27, 27 October 2017
MCherry optimized with three introns for C.elegans
Mcherry, RFP, with three introns added. Mcherry is following the promoter ord-10, which can lead to high expression in AWA. We use this modified mcherry to locate the neuron AWA in C.elegans.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3