Difference between revisions of "Part:BBa K2275008"
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This composite part is composed of R0010, B0034 and K2275002 which encodes glutamate dehydrogenase (GudB) derived from Bacillus subtilis. First, to ensure whether the gene gudB can successfully express in E. coli or not, we used the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to analyze the protein expression of GudB. The SDS-PAGE result is shown below. | This composite part is composed of R0010, B0034 and K2275002 which encodes glutamate dehydrogenase (GudB) derived from Bacillus subtilis. First, to ensure whether the gene gudB can successfully express in E. coli or not, we used the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to analyze the protein expression of GudB. The SDS-PAGE result is shown below. | ||
| − | [[File:SDS- | + | [[File:SDS-PAGE result of GudB.png|350px||centre]] |
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Revision as of 11:07, 27 October 2017
PlacI-B0034-gudB
This composite part is composed of R0010, B0034 and K2275002 which encodes glutamate dehydrogenase (GudB) derived from Bacillus subtilis. First, to ensure whether the gene gudB can successfully express in E. coli or not, we used the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to analyze the protein expression of GudB. The SDS-PAGE result is shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 682
Illegal AgeI site found at 245 - 1000COMPATIBLE WITH RFC[1000]