Difference between revisions of "Part:BBa K2275007"
Line 5: Line 5:
 
This composite part is composed of R0010, B0034 and K2275001 which encodes nitrite reductase derived from E. coli K-12 MG1655 strain.  
 
This composite part is composed of R0010, B0034 and K2275001 which encodes nitrite reductase derived from E. coli K-12 MG1655 strain.  
 
Then we used Griess reagent assay to test the activity of NiR. The principle and mechanism of Griess reaction are shown below.  
 
Then we used Griess reagent assay to test the activity of NiR. The principle and mechanism of Griess reaction are shown below.  
−
[[File:Griess reagent assay mechanism.png|200px|thumb|left|The mechanism of griess reaction]]
+
[[File:Griess reagent assay mechanism.png|200px|left|The mechanism of griess reaction]]
 
When the broth contains nitrite, by adding the Griess reactant which consists of naphthylethylenediamine dihydrochloride, sulphanilamide,  phosphoric acid can form a diazonium salt with nitrite. The diazonium salt will have absorbance at wavelength 545 nm. By the reaction, we can further calculate the nitrate conversion.
 
When the broth contains nitrite, by adding the Griess reactant which consists of naphthylethylenediamine dihydrochloride, sulphanilamide,  phosphoric acid can form a diazonium salt with nitrite. The diazonium salt will have absorbance at wavelength 545 nm. By the reaction, we can further calculate the nitrate conversion.
  

Revision as of 10:29, 27 October 2017


PlacI-B0034-NiR cluster

This composite part is composed of R0010, B0034 and K2275001 which encodes nitrite reductase derived from E. coli K-12 MG1655 strain. Then we used Griess reagent assay to test the activity of NiR. The principle and mechanism of Griess reaction are shown below.

The mechanism of griess reaction

When the broth contains nitrite, by adding the Griess reactant which consists of naphthylethylenediamine dihydrochloride, sulphanilamide, phosphoric acid can form a diazonium salt with nitrite. The diazonium salt will have absorbance at wavelength 545 nm. By the reaction, we can further calculate the nitrate conversion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2093
    Illegal AgeI site found at 551
    Illegal AgeI site found at 947
    Illegal AgeI site found at 2105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401