Difference between revisions of "Part:BBa K2309022:Experience"

Revision as of 09:31, 27 October 2017

Applications of BBa_K2309022

This year we use dot blot method to evaluate part K2309022 work.

The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.

For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as follow:

As picture shows that the concentration of 5-10ug/ml, the LL-37 has highly activity.

We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows:

The result shows

User Reviews

UNIQ3a8aff2095fe07fe-partinfo-00000000-QINU UNIQ3a8aff2095fe07fe-partinfo-00000001-QINU

@@ Line 9: / Line 9: @@
 The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.
-For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC), the result as follow:
+For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as follow:
 https://static.igem.org/mediawiki/parts/5/5c/LL-37_MIC_XJTLU-CHINA.jpeg
 As picture shows that the concentration of 5-10ug/ml, the LL-37 has highly activity.
+We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows:
+https://static.igem.org/mediawiki/parts/6/6c/LL-37_biofilm_XJTLU-CHINA.jpeg
+The result shows
 ===User Reviews===