Difference between revisions of "Part:BBa K2302003"

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<partinfo>BBa_K2302003 short</partinfo>

<partinfo>BBa_K2302003 short</partinfo>

Lpp-OmpA, a signal peptide can be used as an outer-membrane-targeting anchor in E. coli. Proteins fused to OmpA-link can be presented on cell surface.  

Lpp-OmpA, a signal peptide can be used as an outer-membrane-targeting anchor in E. coli. Proteins fused to OmpA-link can be presented on cell surface.  

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===Usage and Biology===

===Usage and Biology===

This sequence encodes a functional peptide that allows surface anchoring of its fused gene product. The peptide consists of (i) the signal sequence and first nine N-terminal amino acids of a mature major lipoprotein of E. coli, (ii) amino acids 46-159 of the outer membrane protein OmpA.[1]

This sequence encodes a functional peptide that allows surface anchoring of its fused gene product. The peptide consists of (i) the signal sequence and first nine N-terminal amino acids of a mature major lipoprotein of E. coli, (ii) amino acids 46-159 of the outer membrane protein OmpA.[1]

===Design===

===Design===

We combined the characteristics of FABP (bind to long-chain fatty acids) and Lpp-OmpA (anchor to the outer membrane of E. coli) in our project. The plasmid pHis contained our restriction sites (HindIII and XbaI). A His tag was added to the C-end of FABP for later western blot to verify the expression of fusion protein Lpp-OmpA-FABP.  

We combined the characteristics of FABP (bind to long-chain fatty acids) and Lpp-OmpA (anchor to the outer membrane of E. coli) in our project. The plasmid pHis contained our restriction sites (HindIII and XbaI). A His tag was added to the C-end of FABP for later western blot to verify the expression of fusion protein Lpp-OmpA-FABP.  

When the whole fusion protein was expressed, the signal peptidewould lead the FABP to the outer membrane of E. coli and anchor in it. So the Follower E in our project could combine fatty acid by the FABP expressed on the outer membrane of E. coli.

When the whole fusion protein was expressed, the signal peptidewould lead the FABP to the outer membrane of E. coli and anchor in it. So the Follower E in our project could combine fatty acid by the FABP expressed on the outer membrane of E. coli.

===Experiment and Result===

===Experiment and Result===