Difference between revisions of "Part:BBa K2381004:Design"
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===Source=== | ===Source=== | ||
| − | + | The light-induced protein nMagHigh is made from the VVD protein family derived from the fungus Neurospora crassa. The Rad2 is made from the dCas9 Protein derived from the Streptococcus pyogenes. | |
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===References=== | ===References=== | ||
Wright A V, Sternberg S H, Taylor D W, et al. Rational design of a split-Cas9 enzyme complex.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(10):2984-2989. | Wright A V, Sternberg S H, Taylor D W, et al. Rational design of a split-Cas9 enzyme complex.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(10):2984-2989. | ||
Revision as of 08:51, 27 October 2017
rad2 + nMagHigh
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1435
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 403
Design Notes
In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a rational strategy of Cas9. According to the different function and spatial position between α-helix lobe and nuclease, we split dCas9 into two parts. Rad1 includes a-helix lobe, Rad2 includes nuclease lobe.
Source
The light-induced protein nMagHigh is made from the VVD protein family derived from the fungus Neurospora crassa. The Rad2 is made from the dCas9 Protein derived from the Streptococcus pyogenes.
References
Wright A V, Sternberg S H, Taylor D W, et al. Rational design of a split-Cas9 enzyme complex.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(10):2984-2989.